Fluorescence in situ hybridization (FISH) has been one of the tried and tested standard methods in molecular pathology for many years: Direct-labelled fluorescence probes bind to its complementary target sequence in the human genome – in situ – in cells and tissue sections, without extraction and amplification of nucleic acids. FISH is a fast and cost-effective method that enables reliable analysis even in very small biopsies with just a few dozen tumor cells. Evaluation of FISH specimens requires a fluorescence microscope.
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